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dcfh da reactive oxygen species detection kit  (Beyotime)
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Beyotime dcfh da reactive oxygen species detection kit
Dcfh Da Reactive Oxygen Species Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dcfh da  (Elabscience Biotechnology)
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AVLE inhibits ferroptosis in HGHF-induced H9c2 cells. Contents of ( A ) Fe 2+ in H9C2 cells. ( B1 ) Representative images and ( B ) fluorescence intensity of <t>DCFH-DA.</t> ( C1 ) Representative images and ( C ) JC-1 fluorescence ratio reflecting MMP. ( D1 ) The images and ( D ) fluorescence intensity of FerroOrange (scale bar = 100 μm); ( E ) representative blot images and the expression levels of GPX4 ( E1 ), FTH-1 ( E2 ), SLC7A11 ( E3 ), TfR1 ( E4 ) and ACSL4 ( E5 ) in H9c2 cells. Data are reported as mean ± SEM (n = 3, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HGHF).
Dcfh Da, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AVLE inhibits ferroptosis in HGHF-induced H9c2 cells. Contents of ( A ) Fe 2+ in H9C2 cells. ( B1 ) Representative images and ( B ) fluorescence intensity of <t>DCFH-DA.</t> ( C1 ) Representative images and ( C ) JC-1 fluorescence ratio reflecting MMP. ( D1 ) The images and ( D ) fluorescence intensity of FerroOrange (scale bar = 100 μm); ( E ) representative blot images and the expression levels of GPX4 ( E1 ), FTH-1 ( E2 ), SLC7A11 ( E3 ), TfR1 ( E4 ) and ACSL4 ( E5 ) in H9c2 cells. Data are reported as mean ± SEM (n = 3, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HGHF).
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fluorescent probe dcfh da assay kit  (Elabscience Biotechnology)
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AVLE inhibits ferroptosis in HGHF-induced H9c2 cells. Contents of ( A ) Fe 2+ in H9C2 cells. ( B1 ) Representative images and ( B ) fluorescence intensity of <t>DCFH-DA.</t> ( C1 ) Representative images and ( C ) JC-1 fluorescence ratio reflecting MMP. ( D1 ) The images and ( D ) fluorescence intensity of FerroOrange (scale bar = 100 μm); ( E ) representative blot images and the expression levels of GPX4 ( E1 ), FTH-1 ( E2 ), SLC7A11 ( E3 ), TfR1 ( E4 ) and ACSL4 ( E5 ) in H9c2 cells. Data are reported as mean ± SEM (n = 3, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HGHF).
Fluorescent Probe Dcfh Da Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reactive oxygen species ros probe dcfh da  (Beijing Solarbio Science)
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AVLE inhibits ferroptosis in HGHF-induced H9c2 cells. Contents of ( A ) Fe 2+ in H9C2 cells. ( B1 ) Representative images and ( B ) fluorescence intensity of <t>DCFH-DA.</t> ( C1 ) Representative images and ( C ) JC-1 fluorescence ratio reflecting MMP. ( D1 ) The images and ( D ) fluorescence intensity of FerroOrange (scale bar = 100 μm); ( E ) representative blot images and the expression levels of GPX4 ( E1 ), FTH-1 ( E2 ), SLC7A11 ( E3 ), TfR1 ( E4 ) and ACSL4 ( E5 ) in H9c2 cells. Data are reported as mean ± SEM (n = 3, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HGHF).
Reactive Oxygen Species Ros Probe Dcfh Da, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2 7 dichlorofuorescindiacetate dcfh da reactive oxygen species fluorometric assay kit  (Elabscience Biotechnology)
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AVLE inhibits ferroptosis in HGHF-induced H9c2 cells. Contents of ( A ) Fe 2+ in H9C2 cells. ( B1 ) Representative images and ( B ) fluorescence intensity of <t>DCFH-DA.</t> ( C1 ) Representative images and ( C ) JC-1 fluorescence ratio reflecting MMP. ( D1 ) The images and ( D ) fluorescence intensity of FerroOrange (scale bar = 100 μm); ( E ) representative blot images and the expression levels of GPX4 ( E1 ), FTH-1 ( E2 ), SLC7A11 ( E3 ), TfR1 ( E4 ) and ACSL4 ( E5 ) in H9c2 cells. Data are reported as mean ± SEM (n = 3, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HGHF).
2 7 Dichlorofuorescindiacetate Dcfh Da Reactive Oxygen Species Fluorometric Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reactive oxygen species assay kit dcfh da  (Beyotime)
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AVLE inhibits ferroptosis in HGHF-induced H9c2 cells. Contents of ( A ) Fe 2+ in H9C2 cells. ( B1 ) Representative images and ( B ) fluorescence intensity of <t>DCFH-DA.</t> ( C1 ) Representative images and ( C ) JC-1 fluorescence ratio reflecting MMP. ( D1 ) The images and ( D ) fluorescence intensity of FerroOrange (scale bar = 100 μm); ( E ) representative blot images and the expression levels of GPX4 ( E1 ), FTH-1 ( E2 ), SLC7A11 ( E3 ), TfR1 ( E4 ) and ACSL4 ( E5 ) in H9c2 cells. Data are reported as mean ± SEM (n = 3, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HGHF).
Reactive Oxygen Species Assay Kit Dcfh Da, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reactive oxygen species assay kit dcfh da  (Beijing Solarbio Science)
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Beijing Solarbio Science reactive oxygen species assay kit dcfh da
AVLE inhibits ferroptosis in HGHF-induced H9c2 cells. Contents of ( A ) Fe 2+ in H9C2 cells. ( B1 ) Representative images and ( B ) fluorescence intensity of <t>DCFH-DA.</t> ( C1 ) Representative images and ( C ) JC-1 fluorescence ratio reflecting MMP. ( D1 ) The images and ( D ) fluorescence intensity of FerroOrange (scale bar = 100 μm); ( E ) representative blot images and the expression levels of GPX4 ( E1 ), FTH-1 ( E2 ), SLC7A11 ( E3 ), TfR1 ( E4 ) and ACSL4 ( E5 ) in H9c2 cells. Data are reported as mean ± SEM (n = 3, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HGHF).
Reactive Oxygen Species Assay Kit Dcfh Da, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AVLE inhibits ferroptosis in HGHF-induced H9c2 cells. Contents of ( A ) Fe 2+ in H9C2 cells. ( B1 ) Representative images and ( B ) fluorescence intensity of <t>DCFH-DA.</t> ( C1 ) Representative images and ( C ) JC-1 fluorescence ratio reflecting MMP. ( D1 ) The images and ( D ) fluorescence intensity of FerroOrange (scale bar = 100 μm); ( E ) representative blot images and the expression levels of GPX4 ( E1 ), FTH-1 ( E2 ), SLC7A11 ( E3 ), TfR1 ( E4 ) and ACSL4 ( E5 ) in H9c2 cells. Data are reported as mean ± SEM (n = 3, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HGHF).
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AVLE inhibits ferroptosis in HGHF-induced H9c2 cells. Contents of ( A ) Fe 2+ in H9C2 cells. ( B1 ) Representative images and ( B ) fluorescence intensity of DCFH-DA. ( C1 ) Representative images and ( C ) JC-1 fluorescence ratio reflecting MMP. ( D1 ) The images and ( D ) fluorescence intensity of FerroOrange (scale bar = 100 μm); ( E ) representative blot images and the expression levels of GPX4 ( E1 ), FTH-1 ( E2 ), SLC7A11 ( E3 ), TfR1 ( E4 ) and ACSL4 ( E5 ) in H9c2 cells. Data are reported as mean ± SEM (n = 3, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HGHF).

Journal: Current Issues in Molecular Biology

Article Title: Protective Effect of Apocynum venetum L. Leaves Extract Against Diabetic Cardiomyopathy: Inhibition of Oxidative Stress and Ferroptosis via Modulation of the Xc − /GSH/GPX4 Axis

doi: 10.3390/cimb48040375

Figure Lengend Snippet: AVLE inhibits ferroptosis in HGHF-induced H9c2 cells. Contents of ( A ) Fe 2+ in H9C2 cells. ( B1 ) Representative images and ( B ) fluorescence intensity of DCFH-DA. ( C1 ) Representative images and ( C ) JC-1 fluorescence ratio reflecting MMP. ( D1 ) The images and ( D ) fluorescence intensity of FerroOrange (scale bar = 100 μm); ( E ) representative blot images and the expression levels of GPX4 ( E1 ), FTH-1 ( E2 ), SLC7A11 ( E3 ), TfR1 ( E4 ) and ACSL4 ( E5 ) in H9c2 cells. Data are reported as mean ± SEM (n = 3, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HGHF).

Article Snippet: Superoxide production, mitochondrial membrane potential (MMP), and ferrous ion (Fe 2+ ) levels were detected using the DCFH-DA (E-BC-K138-F, Elabscience, Wuhan, China), JC-1 (C2006, Beyotime, Shanghai, China), and FerroOrange (F374, Dojindo, Kumamoto, Japan) fluorescent probes, respectively.

Techniques: Fluorescence, Expressing